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Message: <blockquote data-quote="imhotep" data-source="post: 27052082" data-attributes="member: 562115">It's as stated in the post..."The Ct is what's known as the Cycle Threshold - is the number of Cycles needed for the fluorescent signal to cross the threshold above the background."The RT_PCR process requires a temperature cycling reaction called the amplification cycle. For a single test, the amplification cycle is repeated at least 40 times before obtaining the results. The total number of amplification cycles required to detect the virus’ genetic material or viral RNA is defined as the ‘CT’ value or the Cycle Threshold.[ATTACH=full]149842[/ATTACH]Note: Background (Baseline) - very little fluorescence. Cycles between cycles 0 and 15, as shown above is known as the background signal.Expotential Phase - This is when the amount of PCR product doubles for every PCR cycle. This can be seen in cycles 15 and 25 Plateau - amplification slows down  and ultimately plateauThe placement of the line is often determined by the qPCR software, however, users can manually place this line as well.</blockquote>

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