BRAINY

Active member
  • [FONT=Comic Sans MS, Arial, Helvetica]
    image010.gif
    [/FONT]​
    [FONT=Comic Sans MS, Arial, Helvetica]Diagram to show the set-up of the experiment for the actual method [/FONT]​


    [FONT=Comic Sans MS, Arial, Helvetica]The method and the diagram above have been changed according to the improvements made to my pilot method. I used the following actual method: [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 1. Collect and set up the apparatus as illustrated in the diagram above. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 2. Remove the bung from the test tube and place 1cm3 of catalase (yeast) solution into the boiling tube using the 1cm3 syringe. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 3. Replace the bung and fill the beaker with water. Place the end of the delivery tube into the water and make sure that the end of the delivery tube is completely submerged in the water. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 4. Next, take the 10cm3 measuring cylinder and fill it to the top with water. Place your thumb over the end of the measuring cylinder so that no water will escape. Turn the tube upside down and place it over the end of the delivery tube. Make sure that the end of the measuring test tube is underwater before you take your thumb away otherwise the water will escape from the measuring tube. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 5. Now take the syringe out of the bung and fill it with 1cm3 of hydrogen peroxide. Make sure that the hydrogen peroxide is at 10% concentration. Collect the stopwatch. Add the hydrogen peroxide and at the same time start the stopwatch. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 6. Record the volume of oxygen produced by reading it from the measuring test tube for every 30 seconds until the volume of oxygen remains constant for three consecutive readings. Repeat the experiment at least three times to make sure that you get accurate and reliable results. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 7. Wash out the test tube twice to make sure that all of the previous reactants are gone. Then shake the test tube to remove all of the water. Wash out the syringe to ensure that the concentrations are not mixed. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 8. Now repeat the experiment using 0% (water), 2%, 4%, 6%, 8% and 10% concentrations of H2O2. Dilute the H2O2 with water. For example, to make 8% concentration, dilute 0.8cm3 of H2O2 with 0.2cm3 of water. Repeat the above but with different volumes of water and H2O2 for the other solutions. [/FONT]

    enzyme.jpg


    [FONT=Comic Sans MS, Arial, Helvetica] 9. Make sure that each experiment is repeated three times to make the results reliable. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 10. When repeating the experiment with diluted concentrations make that you gently swirl the test tube with the solution inside to make sure that you thoroughly dilute the H2O2. [/FONT]


    [FONT=Comic Sans MS, Arial, Helvetica]Safety [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica]Make sure that goggles and laboratory coats are worn whenever syringing. Make sure that a damp cloth is nearby to mop up any spilt hydrogen peroxide. [/FONT]



    [FONT=Comic Sans MS, Arial, Helvetica]Apparatus[/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Test tube rack. [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Stopwatch [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Boiling tube [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Beaker [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ ‘S’ shape delivery tube [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Bung with inserted needle [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 2 ´ Syringe [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 20cm3 of hydrogen peroxide at 10% concentration [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 20cm3 of catalase (yeast) solution at 5% concentration [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Test tube with cm3 divisions of at least 0.2cm3 [/FONT]


    [FONT=Comic Sans MS, Arial, Helvetica]WHAT TO DO NEXT: [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica]Now you have read this plan you need to carry out the practical. [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica]Your teacher will explain exactly how to go about this, but you can use the check-list below to keep a track of your progress. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] o Carry out the pilot method to familiarise yourself with the apparatus.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Carry out the main practical and record all results.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Draw up a ‘Perfect Table’ of results.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Construct a ‘Perfect Graph’ and Analyse the results in detail.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Evaluate the method – looking for errors and improvements.[/FONT]
     
    Last edited:

    xXxHaNzxXx

    Member
    Jun 27, 2006
    3,911
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    0
    Djpata said:
    aioo paww:(:lol: well finish ur a/l'z 1st N then u'll be able to cook soo many new recipes from ur own nee;):P and also wish u all da very best 4 ur o/l'z study hard nangiya:D
    ehehehe yeee..id prbly av 2 mke a hosp 4 al da ppl hu eat ma foood..:( :sorry:
    Thankoo..hugz
     

    xXxHaNzxXx

    Member
    Jun 27, 2006
    3,911
    0
    0
    BRAINY said:
    [FONT=Comic Sans MS, Arial, Helvetica]
    image010.gif
    [/FONT]​
    [FONT=Comic Sans MS, Arial, Helvetica]Diagram to show the set-up of the experiment for the actual method [/FONT]​


    [FONT=Comic Sans MS, Arial, Helvetica]The method and the diagram above have been changed according to the improvements made to my pilot method. I used the following actual method: [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 1. Collect and set up the apparatus as illustrated in the diagram above. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 2. Remove the bung from the test tube and place 1cm3 of catalase (yeast) solution into the boiling tube using the 1cm3 syringe. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 3. Replace the bung and fill the beaker with water. Place the end of the delivery tube into the water and make sure that the end of the delivery tube is completely submerged in the water. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 4. Next, take the 10cm3 measuring cylinder and fill it to the top with water. Place your thumb over the end of the measuring cylinder so that no water will escape. Turn the tube upside down and place it over the end of the delivery tube. Make sure that the end of the measuring test tube is underwater before you take your thumb away otherwise the water will escape from the measuring tube. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 5. Now take the syringe out of the bung and fill it with 1cm3 of hydrogen peroxide. Make sure that the hydrogen peroxide is at 10% concentration. Collect the stopwatch. Add the hydrogen peroxide and at the same time start the stopwatch. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 6. Record the volume of oxygen produced by reading it from the measuring test tube for every 30 seconds until the volume of oxygen remains constant for three consecutive readings. Repeat the experiment at least three times to make sure that you get accurate and reliable results. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 7. Wash out the test tube twice to make sure that all of the previous reactants are gone. Then shake the test tube to remove all of the water. Wash out the syringe to ensure that the concentrations are not mixed. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 8. Now repeat the experiment using 0% (water), 2%, 4%, 6%, 8% and 10% concentrations of H2O2. Dilute the H2O2 with water. For example, to make 8% concentration, dilute 0.8cm3 of H2O2 with 0.2cm3 of water. Repeat the above but with different volumes of water and H2O2 for the other solutions. [/FONT]

    enzyme.jpg


    [FONT=Comic Sans MS, Arial, Helvetica] 9. Make sure that each experiment is repeated three times to make the results reliable. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] 10. When repeating the experiment with diluted concentrations make that you gently swirl the test tube with the solution inside to make sure that you thoroughly dilute the H2O2. [/FONT]


    [FONT=Comic Sans MS, Arial, Helvetica]Safety [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica]Make sure that goggles and laboratory coats are worn whenever syringing. Make sure that a damp cloth is nearby to mop up any spilt hydrogen peroxide. [/FONT]



    [FONT=Comic Sans MS, Arial, Helvetica]Apparatus[/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Test tube rack. [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Stopwatch [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Boiling tube [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Beaker [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ ‘S’ shape delivery tube [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Bung with inserted needle [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 2 ´ Syringe [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 20cm3 of hydrogen peroxide at 10% concentration [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 20cm3 of catalase (yeast) solution at 5% concentration [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] · 1 ´ Test tube with cm3 divisions of at least 0.2cm3 [/FONT]


    [FONT=Comic Sans MS, Arial, Helvetica]WHAT TO DO NEXT: [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica]Now you have read this plan you need to carry out the practical. [/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica]Your teacher will explain exactly how to go about this, but you can use the check-list below to keep a track of your progress. [/FONT]

    [FONT=Comic Sans MS, Arial, Helvetica] o Carry out the pilot method to familiarise yourself with the apparatus.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Carry out the main practical and record all results.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Draw up a ‘Perfect Table’ of results.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Construct a ‘Perfect Graph’ and Analyse the results in detail.[/FONT]
    [FONT=Comic Sans MS, Arial, Helvetica] o Evaluate the method – looking for errors and improvements.[/FONT]
    :shocked: :shocked: :shocked: Thank You sooo sooo sooo sooo sooo much..ur a life saver.....hugz