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Message: <blockquote data-quote="imhotep" data-source="post: 28198104" data-attributes="member: 562115">Researchers at the University of Illinois Urbana-Champaign has demonstrated the ability pf DNA Net structures to detect and impede Covid virus in human cell cultures. They have shown that  their DNA nets effectively targeted the spike protein and were able to detect the virus at very low levels, equivalent to the sensitivity of gold-standard PCR tests that can take a day or more.The technique does not need any special preparation or equipment, and can be performed at room temperature, so all a user would do is mix the sample with the solution and read it. The researchers estimated in their study that the method would cost $1.26 per test. Also it has the ability to detect the entire virus, which is still infectious, and distinguish it from fragments that may not be infectious anymore.In addition, the DNA Nets inhibited the virus's spread in live cell cultures, with the antiviral activity increasing with the size of the DNA net scaffold. This points to DNA structures' potential as therapeutic agent.ABSTRACT:"We present a net-shaped DNA nanostructure (called “DNA Net” herein) design strategy for selective recognition and high-affinity capture of intact SARS-CoV-2 virions through spatial pattern-matching and multivalent interactions between the aptamers (targeting wild-type spike-RBD) positioned on the DNA Net and the trimeric spike glycoproteins displayed on the viral outer surface. Carrying a designer nanoswitch, the DNA Net-aptamers release fluorescence signals upon virus binding that are easily read with a handheld fluorimeter for a rapid (in 10 min), simple (mix-and-read), sensitive (PCR equivalent), room temperature compatible, and inexpensive (∼$1.26/test) COVID-19 test assay. The DNA Net-aptamers also impede authentic wild-type SARS-CoV-2 infection in cell culture with a near 1 × 10^3-fold enhancement of the monomeric aptamer. Furthermore, our DNA Net design principle and strategy can be customized to tackle other life-threatening and economically influential viruses like influenza and HIV, whose surfaces carry class-I viral envelope glycoproteins like the SARS-CoV-2 spikes in trimeric forms."[ATTACH=full]185166[/ATTACH]The National Institutes of Health supported this work through the Rapid Acceleration of Diagnostics program.</blockquote>

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