Microchip based real-time PCR techniques with the extremely fast heating and cooling device performs rapid amplification and analysis in micro-volumes. Lumex Instruments Canada has created an accurate, cost-effective, and fast Microchip RT-PCR Influenza and COVID-19 detection method from upper respiratory tract samples (swabs).
These come with pre-loaded microchip kit with the primers and probes lyophilized in the microchip for testing of SARS-CoV-2 and the Influenza A & B viruses. N1 primers-probe target the nucleocapsid gene (N) gene of SARS-CoV-2 virus, while the Inf-A primers-probe are targeted to focus on matrix (M1) gene of Influenza A virus and Inf-B primers-probe target nonstructural 2 (NS2) gene of Influenza B virus.
The current PCR assays need large volumes of reagents (20 μl reaction) against this Microchip RT-PCR Influenza and COVID-19 detection need only much less (1.2 μl reaction). Also lyophilized PCR reagents in the microchips will boost the reliability of laboratory analysis considerably.
These come with pre-loaded microchip kit with the primers and probes lyophilized in the microchip for testing of SARS-CoV-2 and the Influenza A & B viruses. N1 primers-probe target the nucleocapsid gene (N) gene of SARS-CoV-2 virus, while the Inf-A primers-probe are targeted to focus on matrix (M1) gene of Influenza A virus and Inf-B primers-probe target nonstructural 2 (NS2) gene of Influenza B virus.
The current PCR assays need large volumes of reagents (20 μl reaction) against this Microchip RT-PCR Influenza and COVID-19 detection need only much less (1.2 μl reaction). Also lyophilized PCR reagents in the microchips will boost the reliability of laboratory analysis considerably.