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PCR technology moves on....
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<blockquote data-quote="imhotep" data-source="post: 26897052" data-attributes="member: 562115"><p>Microchip based real-time PCR techniques with the extremely fast heating and cooling device performs rapid amplification and analysis in micro-volumes. Lumex Instruments Canada has created an accurate, cost-effective, and fast <strong>Microchip RT-PCR Influenza and COVID-19 detection method</strong> from upper respiratory tract samples (swabs).</p><p></p><p>These come with pre-loaded microchip kit with the primers and probes lyophilized in the microchip for testing of SARS-CoV-2 and the Influenza A & B viruses. N1 primers-probe target the nucleocapsid gene (N) gene of SARS-CoV-2 virus, while the Inf-A primers-probe are targeted to focus on matrix (M1) gene of Influenza A virus and Inf-B primers-probe target nonstructural 2 (NS2) gene of Influenza B virus.</p><p></p><p>The current PCR assays need large volumes of reagents (20 μl reaction) against this Microchip RT-PCR Influenza and COVID-19 detection need only much less (1.2 μl reaction). Also lyophilized PCR reagents in the microchips will boost the reliability of laboratory analysis considerably.</p></blockquote><p></p>
[QUOTE="imhotep, post: 26897052, member: 562115"] Microchip based real-time PCR techniques with the extremely fast heating and cooling device performs rapid amplification and analysis in micro-volumes. Lumex Instruments Canada has created an accurate, cost-effective, and fast [B]Microchip RT-PCR Influenza and COVID-19 detection method[/B] from upper respiratory tract samples (swabs). These come with pre-loaded microchip kit with the primers and probes lyophilized in the microchip for testing of SARS-CoV-2 and the Influenza A & B viruses. N1 primers-probe target the nucleocapsid gene (N) gene of SARS-CoV-2 virus, while the Inf-A primers-probe are targeted to focus on matrix (M1) gene of Influenza A virus and Inf-B primers-probe target nonstructural 2 (NS2) gene of Influenza B virus. The current PCR assays need large volumes of reagents (20 μl reaction) against this Microchip RT-PCR Influenza and COVID-19 detection need only much less (1.2 μl reaction). Also lyophilized PCR reagents in the microchips will boost the reliability of laboratory analysis considerably. [/QUOTE]
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