කොවිඩ් 19 හැදිලා සුව වෙලා (pcr නේගටිව්) කෙනෙකුගෙන් අනෙක් අයෙක්ට බෝ වීමේ සම්භාවිතාවක් තියෙනවද? එහෙම තියෙනවනම් සුව වෙලා දවස් කියක් වගේ යනකම්ද?
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මොකක්ද මේ CT Value (COVID 19 PCR Report)
- Thread starter divoomdelux
- Start date
This is an extract from a post I made in 14 November 2020 - on the interpretation of the Ct values... This figure clearly shows how the Ct values can reflect on infectivity.
<snip>
On a previous post on a Covid Megathread, I have explained in detail how a RT-PCR is done. The Ct value does play a part. The Ct is what's known as the Cycle Threshold - is the number of Cycles needed for the fluorescent signal to cross the threshold above the background. A PCR is done usually to a max of 40 cycles...
Cts < 29 are strong positive reactions indicative of abundant target nucleic acid
Cts of 30-37 are positive reactions indicative of moderate amounts of target nucleic acid
Cts of 38-40 are weak reactions indicative of minimal amounts of target nucleic acid which could represent an infection state or environmental contamination.
So a high CT value is dubious. You cannot rely on Ct after 37 at all. In which case you do another PCR test a few days later. These can be false positives.
A team of scientists recently did a study more than 3000 cases the graph below shows the results. Ct against infectvity. They did a PCR test and also they did a viral culture using the same swab to independently grow the virus to confirm. The number of Samples N is the PCR test, The number of Samples n is the Virus detected in the culture.
One can easily see at high Ct values the results are inaccurate. The Global percentage value graph is 100% at low Ct values and then keeps on falling down to zero at Ct around 36. This is well known but only probably not known by an average person.
Also studies done in several countries (most recently in Brazil) has shown that nearly 35% of patients Ct value of 25 or below were more likely to have severe disease or die.
<snip>
<snip>
On a previous post on a Covid Megathread, I have explained in detail how a RT-PCR is done. The Ct value does play a part. The Ct is what's known as the Cycle Threshold - is the number of Cycles needed for the fluorescent signal to cross the threshold above the background. A PCR is done usually to a max of 40 cycles...
Cts < 29 are strong positive reactions indicative of abundant target nucleic acid
Cts of 30-37 are positive reactions indicative of moderate amounts of target nucleic acid
Cts of 38-40 are weak reactions indicative of minimal amounts of target nucleic acid which could represent an infection state or environmental contamination.
So a high CT value is dubious. You cannot rely on Ct after 37 at all. In which case you do another PCR test a few days later. These can be false positives.
A team of scientists recently did a study more than 3000 cases the graph below shows the results. Ct against infectvity. They did a PCR test and also they did a viral culture using the same swab to independently grow the virus to confirm. The number of Samples N is the PCR test, The number of Samples n is the Virus detected in the culture.
One can easily see at high Ct values the results are inaccurate. The Global percentage value graph is 100% at low Ct values and then keeps on falling down to zero at Ct around 36. This is well known but only probably not known by an average person.
Also studies done in several countries (most recently in Brazil) has shown that nearly 35% of patients Ct value of 25 or below were more likely to have severe disease or die.
<snip>
Ow body aches enwa, panadol arenna wena dewal ganna yanna epa, oya diclofenac wage dewal ganna eka recommend karanne nehe,
Den dys keeyak wnwada??
Eka mchn depend wenawa initial CT value eka matha, ct value eka godak adu nam tika kalauak yanakal infective wenna puluwan, atleast thawa 14 days wath parissam wela inna, dys 14 iwara wela gihillath RAT kaloth positive wena aya innawa..
Yeah, tfs
------ Post added on Aug 13, 2021 at 2:22 PM
can you explain CT value more. What is the thing that is measuared(scientifically) and how it relate to severity of the spread to others
bohoma sthuthi information valata.. matath covid positive vuna 1st of Aug. hotel qurantine vela dan gedara inne.. thama sema vage thiyanava lavata kassath ekka.. thava agata mahansi vage saren sare vada karanakota.
Zincovit gena thamai bro mama kiwwe, eka gaththa kiyala athi wedak nehe, gaththa kiyla haniyakuth nehe.
Ehema nehe, bt vaccine eke efficacy eka adu wenwa
Sure bro, ill explain in more, hopefully tmrw ill make a post fr u, bit hard to explain in sinhala
And it does not relate to the severity, it relates to the infectivity
Sathiyak dekak yanakal thiyei bro, isolate wela inna gedarath
------ Post added on Aug 13, 2021 at 3:23 PM
Please read the first para on the extract I copied. If you could locate my post on the PCR that explains how the PCR is done.
Very briefly, a PCR is done to quantify a target sequence and it involves amplifcation of the target in real-time via a target-specific fluorescent signal emitted during amplification.
The process is just like a molecular photcopier. To amplify a small segment of DNA using PCR, the sample is first heated so that it separates into two pieces of single-stranded DNA. Next, an enzyme called "Taq polymerase" is used to synthesize- two new strands of DNA, resulting in the duplication of the original DNA, with each of the new molecules containing one old and one new strand of DNA. Then each of these strands can be used to create two new copies, and so on, and so on. This process is done to 40 times max (cycles) as I mentioned before.
However, in practice even though the PCR dyes and probes are sequence specific, there's considerable background fluorescence occurs. This signal to noise issue is addressed by two things - a threshold line and the Ct value.
Threshold line is the level of detection point where the reaction reaches a fluorescent intensity above background levels and the Ct value is the PCR cycle number at which your sample’s reaction curve intersects the threshold line.
How it relates to infectivity is the graph on my post....
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Ohh, no no bro, vaccine eken RAT wath , PCR wath positive wenne nehe,, meka sampoorna weradi mathayak..
Weeks deka inna mchn, himeeta aduwei
------ Post added on Aug 13, 2021 at 4:15 PM
ubata positive unada? ehenan corona Positive ,Vaccine gahuwa kiyala positive wenne na..
Ekiynne positive une 6th wageda, ehenan 20th gahanna epa bro, egollonta guideline ekata anuwa gahanneth behe, gahanna wenne thawa dsy 22kin passe.. eka awulak wenne nehe
Thanks for share with us...
covid hadunama sema yanna beheth warga denawada? thiyenawanam dr kenekge guidance anuwada bonna one.
covid hadunama sema yanna beheth warga denawada? thiyenawanam dr kenekge guidance anuwada bonna one.
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